EVIDENCE THAT MORE THAN ONE GENE ENCODES NORMAL-ALKANE-INDUCIBLE CYTOCHROME-P-450S IN CANDIDA-MALTOSA, FOUND BY 2-STEP GENE DISRUPTION

被引：25

作者：

OHKUMA, M ^{[1
]}

HIKIJI, T ^{[1
]}

TANIMOTO, T ^{[1
]}

SCHUNCK, WH ^{[1
]}

MULLER, HG ^{[1
]}

YANO, K ^{[1
]}

TAKAGI, M ^{[1
]}

机构：

[1] CENT INST MOLEC BIOL,O-1115 BERLIN,GERMANY

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1991年 / 55卷 / 07期

关键词：

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENES; HOST-VECTOR SYSTEM; SACCHAROMYCES-CEREVISIAE; YEAST; PROTEINS; CONSTRUCTION; TROPICALIS; GENOME; CELLS;

D O I：

10.1080/00021369.1991.10870878

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

An n-alkane-assimilating yeast, Candida maltosa, has a diploid genome. Probed with the previously isolated gene of n-alkane-inducible cytochrome P-450 (P-450alk), its allelic gene had been isolated, its nucleotides sequenced, and the interallelic divergence examined. Using one of the allelic genes, we disrupted the two alleles of the cytochrome P-450alk gene by a two-step gene disruption system. Surprisingly, the disruptant still assimilated n-alkane and contained n-alkane-inducible cytochrome P-450. This result indicates that, other than the disrupted two alleles, there is at least one other gene that encodes an n-alkane-inducible cytochrome P-450.

引用

页码：1757 / 1764

页数：8

共 26 条

[1] PLOIDY IN THE ASPOROGENOUS YEAST CANDIDA-MALTOSA, ISOLATION OF ITS AUXOTROPHIC MUTANTS AND THEIR CELL-FUSION [J].