GLUCOCORTICOID INDUCTION OF CRE-BINDING PROTEIN ISOFORM MESSENGER-RNAS IN RAT C6 GLIOMA-CELLS

被引：21

作者：

JUNGMANN, RA ^{[1
]}

WANG, XS ^{[1
]}

MILKOWSKI, DM ^{[1
]}

SHORT, ML ^{[1
]}

机构：

[1] NORTHWESTERN UNIV,SCH MED,CTR CANC,CHICAGO,IL 60611

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/20.4.825

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian cells express several distinct isoforms of transcription factor CREB (cAMP-responsive element binding protein). At least two forms, alpha- and DELTA-CREB, arise through alternative splicing of the CREB gene transcript. In this communication we demonstrate that the mRNAs of several CREB isoforms are expressed in rat C6 glioma cells and that the intracellular levels of these mRNAs are markedly induced by the synthetic glucocorticoid dexamethasone. Nuclear run-off assays show that the induction occurs, at least in part, through a transcriptional mechanism. The enhanced cellular levels of CREB mRNAs are accompanied by increased CREB protein and CRE-binding activity of nuclear extracts as evaluated by immunoblot and Southwestern blot assays.

引用

页码：825 / 829

页数：5

共 28 条

[1] NEGATIVE REGULATION BY GLUCOCORTICOIDS THROUGH INTERFERENCE WITH A CAMP RESPONSIVE ENHANCER [J].