IDENTIFICATION OF CHICKEN (GALLUS-DOMESTICUS) ADIPOCYTE PLASMA-MEMBRANE AND DIFFERENTIATION SPECIFIC PROTEINS USING SDS PAGE AND WESTERNBLOTTING

被引:10
作者
BUTTERWITH, SC
KESTIN, S
GRIFFIN, HD
BEATTIE, J
FLINT, DJ
机构
[1] UNIV BRISTOL,DEPT MEAT ANIM SCI,BRISTOL BS18 7DY,ENGLAND
[2] HANNAH RES INST,AYR KA6 5HL,SCOTLAND
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1992年 / 101卷 / 1-2期
关键词
D O I
10.1016/0305-0491(92)90170-V
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. Affinity-purified adipocyte membrane proteins were used to raise antisera in two sheep. 2. Using one of the antisera 15 proteins were identified as being adipocyte specific by comparison on Western blots of plasma membrane proteins from various tissues. 3. Of these 15 proteins eight appeared to be present only in mature adipocytes and not in the adipocyte precursor. 4. In the presence of guinea pig complement the two antisera raised were cytotoxic to adipocytes and their precursors. 5. Characterization and further study of these adipocyte differentiation specific proteins will provide valuable information about the process of adipocyte differentiation.
引用
收藏
页码:147 / 151
页数:5
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