OPTIMIZATION OF THE MAGNETIC BEAD ANTIGEN CAPTURE ENZYME IMMUNE ASSAY FOR THE DETECTION OF CIRCULATING ANODIC ANTIGENS IN MIXED SCHISTOSOMA INFECTIONS

被引：7

作者：

NDHLOVU, P

CADMAN, H

GUNDERSEN, SG

VENNERVALD, B

FRIIS, H

CHRISTENSEN, N

MUTASA, G

HAAGENSEN, I

CHANDIWANA, SK

DEELDER, AM

机构：

[1] UNIV ZIMBABWE,DEPT BIOCHEM,HARARE,ZIMBABWE

[2] UNIV OSLO,ULLEVAAL HOSP,RES FORUM,ULLEVAAL CTR INT MED,N-0407 OSLO,NORWAY

[3] DANISH BILHARZIASIS LAB,DK-2920 CHARLOTTENLUND,DENMARK

[4] LEIDEN UNIV,FAC MED,PARASITOL LAB,2300 RC LEIDEN,NETHERLANDS

来源：

ACTA TROPICA | 1995年 / 59卷 / 03期

关键词：

SCHISTOSOMA HAEMATOBIUM; SCHISTOSOMA MANSONI; MBAC-EIA; MAGNETIC BEADS; CIRCULATING ANODIC ANTIGEN; IMMUNODIAGNOSIS;

D O I：

10.1016/0001-706X(95)00083-Q

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

In the present study, simplification and adaptation of the Magnetic Bead Antigen Capture Enzyme Immune Assay (MBAC-EIA) technique for detection of circulating anodic antigens (CAA) under held conditions was achieved. It was shown that the assay could be performed successfully within the broad temperature range of 18-37 degrees C. The slightly lower sensitivity observed at low temperatures could be adjusted for by prolonging the incubation period. Shaking the plate by hand was as good as automatic mechanical shaking, aspiration of the supernatant before the addition of conjugate was not necessary, and the use of whole blood and serum offered similar assay sensitivity. Furthermore incubation times could be considerably shortened without loss of sensitivity. A major advantage of the MBAC-EIA was that the beads, after elution of bound components, were found to be reusable. The study also showed that the sensitivity of the MBAC-EIA technique in diagnosis of schistosomiasis in a Zimbabwean community endemic for both urinary and intestinal schistosomiasis, was 94%.

引用

页码：223 / 235

页数：13

共 24 条

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