HIGH-FREQUENCY TRANSFORMATION OF ALCALIGENES-EUTROPHUS PRODUCING POLY-BETA-HYDROXYBUTYRIC ACID BY ELECTROPORATION

被引：30

作者：

PARK, HC

LIM, KJ

PARK, JS

LEE, YH

HUH, TL

机构：

[1] Department of Genetic Engineering, College of Natural Sciences Kyungpook National University, Taegu

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 01期

关键词：

D O I：

10.1007/BF00152996

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A strain of Alcaligenes eutrophus producing poly-beta-hydroxybutyric acid was successfully transformed by the electroporation. The plasmid used was a broad host range plasmid pKT230 conferring kanamycin resistance. The optimum yield of transformant was 0.8x10(2)/mu g DNA when 50 mu l competent cells at 10(10)/ml were pulsed by 11.5 kV/cm for 5 ms with 1 mu g DNA. Plasmid DNA in the A. eutrophus transformant was stably maintained as a monomeric structure.

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页码：31 / 34

页数：4

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