SIMPLE TECHNIQUE FOR INVIVO OBSERVATION OF SCE IN MOUSE ASCITES TUMOR AND SPERMATOGONIAL CELLS

被引：37

作者：

KANDA, N ^{[1
]}

KATO, H ^{[1
]}

机构：

[1] NATL INST GENET,DEPT CYTOGENET,MISHIMA,SHIZUOKA 411,JAPAN

来源：

EXPERIMENTAL CELL RESEARCH | 1979年 / 118卷 / 02期

关键词：

D O I：

10.1016/0014-4827(79)90173-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A simplified technique to observe sister chromatid exchange (SCE) in ascites tumor cells and spermatogonial cells of the mouse was described. The technique consisted of a single intraperitoneal injection of 5-bromo-2′-deoxyuridine (BUdR) absorbed on activated charcoal that assured continuous release of BUdR throughout two rounds of the replication period. The minimum dose of BUdR to detect SCE was found to be 5 mg/body, the SCE frequency being 4.50 - 4.90/ cell in tumor cells and 1.30 - 1.88/cell in spermatogonia, respectively. © 1979.

引用

页码：431 / 434

页数：4

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