CHARACTERIZATION OF THE PHOSPHORYL-NITROGEN LINKAGES IN HISTONE-H4

被引：2

作者：

BRUEGGER, BB

DELANGE, RJ

SMITH, RA

LIN, YC

机构：

[1] UNIV CALIF LOS ANGELES,INST MOLEC BIOL,LOS ANGELES,CA 90024

[2] NATL TAIWAN UNIV,DEPT CHEM,TAIPEI,TAIWAN

来源：

JOURNAL OF THE CHINESE CHEMICAL SOCIETY | 1979年 / 26卷 / 01期

关键词：

D O I：

10.1002/jccs.197900002

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In earlier work we nave described and partially characterized two histone kinases from regenerating rat liver and other tissues which catalyze the transfer of the γ‐phosphoryl group from ATP to histones. The histone phosphates thus formed were observed to be acid‐labile and base‐stable. In the present study we report on the specificity of one of these enzymes, namely, the kinase which is optimally active at pH 9. This enzyme appears to be relatively specific for the two histidine residues of histone H4. These histidines occur at positions 18 and 75, and both are phosphorylated. However, when regenerating rat liver was the source of enzyme, the product was 1‐phosphohistidine, whereas the enzyme from Walker‐256 carcinosarcoma catalyzed the formation of 3‐phosphohistidine. Copyright © 1979 The Chemical Society Located in Taipei & Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim, Germany

引用

页码：5 / 10

页数：6

共 15 条

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