A LUMINOMETRIC METHOD FOR THE DETERMINATION OF ATP AND PHOSPHOCREATINE IN SINGLE HUMAN SKELETAL-MUSCLE FIBERS

被引：35

作者：

WIBOM, R

SODERLUND, K

LUNDIN, A

HULTMAN, E

机构：

[1] HUDDINGE UNIV HOSP,DEPT CLIN CHEM 2,S-14186 HUDDINGE,SWEDEN

[2] HUDDINGE UNIV HOSP,DEPT CHEM,S-14186 HUDDINGE,SWEDEN

[3] HUDDINGE UNIV HOSP,CLIN RES CTR,S-14186 HUDDINGE,SWEDEN

来源：

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1991年 / 6卷 / 02期

关键词：

ATP; LUMINESCENCE; PHOSPHOCREATINE; SINGLE FIBERS;

D O I：

10.1002/bio.1170060210

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive method for the analysis of ATP and phosphocreatine (PCr) in single human skeletal muscle fibres is described. Muscle tissue was freeze-dried and single fibres were dissected free with the aid of low-power microscopy. The fibres were then extracted in trichloroacetic acid and neutralized with KHCO3. The assay is based on the continuous monitoring of light produced as a result of ATP degradation in the firefly luciferase reaction. PCr is measured as the amount of ATP formed in the creatine kinase reaction. The coefficient of variation was less than 4 % for both ATP and PCr determination. The amount of tissue required for the assay is approximately 0.5-mu-g (dry weight). The assay showed good agreement with spectrophotometric and high-performance liquid chromatographic (HPLC) measurements made upon extracts of whole muscle tissue.

引用

页码：123 / 129

页数：7

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