CROSS-REACTIVITY BETWEEN ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPES COMPARING DIFFERENT ANTIGENS AND SEROLOGICAL TESTS

被引:22
作者
GUTIERREZ, CB
TASCON, RI
VAZQUEZ, JA
FERRI, EFR
机构
[1] Unidad de Microbiología e Inmunología, Departamento de Patología Animal, Sanidad Animal
关键词
D O I
10.1016/0034-5288(91)90129-C
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Several antigens were prepared from suspensions of reference strains of the 12 serotypes of Actinobacillus pleuropneumoniae (saline extract [SE], capsular extract [CE], whole cell suspension [WCS], boiled extract [BE], and autoclaved cell antigen [ACA]). The cross reactions between each antigen and the antisera against reference strains of the other 11 serotypes were compared by using the complement fixation test, ELISA and the indirect haemagglutination test. ACA produced the most cross reactions, which, in some serotypes, took place in all the antisera tested. BE produced fewer cross reactions, but these were more abundant than those obtained with SE, CE or WCS. The least cross reactivity occurred with SE in the indirect haemagglutination test. This test is, therefore, the most reliable method for serotyping field strains of A pleuropneumoniae.
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页码:308 / 310
页数:3
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