IMMUNOLOGICAL DEMONSTRATION OF EPSILON-PKC - MURINE TISSUE DISTRIBUTION, ONTOGENY, CELLULAR-LOCALIZATION AND TRANSLOCATION

被引：12

作者：

GSCHWENDT, M

LEIBERSPERGER, H

RINCKE, G

MARKS, F

机构：

[1] Institute of Biochemistry, German Cancer Research Center, D-6900 Heidelberg

来源：

FEBS LETTERS | 1991年 / 290卷 / 1-2期

关键词：

EPSILON-PROTEIN KINASE-C; ANTISERUM; MOUSE BRAIN; ONTOGENY; TRANSLOCATION;

D O I：

10.1016/0014-5793(91)81239-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An antiserum raised against an epsilon-PKC-specific peptide recognizes epsilon-PKC with an apparent molecular weight of 97 kDa in cytosol of mouse brain. No cross-reaction with alpha,beta,gamma-PKC or the sigma-PKC-like p76-kinase is observed. Epsilon-PKC is mainly present in brain. Just traces of this PKC isoenzyme can be detected in some other murine tissues. Ontogenetic studies indicated that the amount of epsilon-PKC in murine brain increases constantly and reaches a maximal level at day 7 after birth. Upon TPA activation epsilon-PKC is translocated from the cytosol to the particulate fraction in a brain homogenate.

引用

页码：115 / 118

页数：4

共 20 条

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