FOOT-AND-MOUTH-DISEASE - DETECTION OF ANTIBODIES IN CATTLE SERA BY BLOCKING ELISA

被引：21

作者：

SORENSEN, KJ

MADEKUROZWA, RL

DAWE, P

机构：

[1] Veterinary Research Laboratory, Harare

来源：

VETERINARY MICROBIOLOGY | 1992年 / 32卷 / 3-4期

关键词：

D O I：

10.1016/0378-1135(92)90148-M

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A blocking ELISA was developed for the detection of antibodies to foot-and-mouth disease virus SAT1, SAT2 and SAT3 and for the quantification of antibodies on a single dilution of serum. The avidin-biotin system was used. The test was compared with the liquid-phase ELISA executed at the World Reference Laboratory for foot-and-mouth disease. It was found to have favourable logistics and combined high specificity with high sensitivity. The quantitative test using a single dilution of serum was resource saving and proved to be a reliable and precise method for the assessment of antibody levels.

引用

页码：253 / 265

页数：13

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