EFFECT OF TRANSFORMING GROWTH-FACTOR-BETA ON COLLAGEN TYPE-VI EXPRESSION IN HUMAN DERMAL FIBROBLASTS

被引：42

作者：

HECKMANN, M

AUMAILLEY, M

CHU, ML

TIMPL, R

KRIEG, T

机构：

[1] UNIV COLOGNE,DERMATOL KLINKEN,DEPT DERMATOL,JOSEPH STELZMANN STR 9,W-5000 COLOGNE 41,GERMANY

[2] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

[3] UNIV MUNICH,DERMATOL KLINIKEN,W-8000 MUNICH 2,GERMANY

[4] THOMAS JEFFERSON UNIV,JEFFERSON INST MOLEC MED,DEPT BIOCHEM,PHILADELPHIA,PA 19107

[5] THOMAS JEFFERSON UNIV,JEFFERSON INST MOLEC MED,DEPT MOLEC BIOL,PHILADELPHIA,PA 19107

[6] THOMAS JEFFERSON UNIV,JEFFERSON INST MOLEC MED,DEPT DERMATOL,PHILADELPHIA,PA 19107

来源：

FEBS LETTERS | 1992年 / 310卷 / 01期

关键词：

D O I：

10.1016/0014-5793(92)81151-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Steady-state mRNA levels and protein synthesis of collagen type VI were determined after stimulation of human dermal fibroblasts with transforming growth factor-beta (TGF-beta). While there was a 227% increase in the alpha-3(VI) unit mRNA at maximal TGF-beta concentration, alpha-1(VI) and alpha-2(VI) subunit mRNA levels remained unchanged. Concomitantly collagen type VI immuno-reactive material increased up to 172% of controls in cell culture medium and cell layer extracts. Regulation of alpha-3(VI) gene expression is therefore critical for the control of collagen type VI synthesis and determines the deposition of collagen type VI heterotrimeric molecules.

引用

页码：79 / 82

页数：4

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