ISOLATING RNA FROM WHOLE-BLOOD - THE DAWN OF RNA-BASED DIAGNOSIS

被引：47

作者：

MACFARLANE, DE ^{[1
]}

DAHLE, CE ^{[1
]}

机构：

[1] UNIV IOWA,IOWA BIOTECHNOL CORP,INST TECHNOL INNOVAT,IOWA CITY,IA 52242

来源：

NATURE | 1993年 / 362卷 / 6416期

关键词：

D O I：

10.1038/362186a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A novel cationic surfactant solution is used to lyse blood cells and precipitate RNA and DNA. The RNA is recovered by extracting the pellet with a small volume of guanidinium isothiocyanate or formamide.

引用

页码：186 / 188

页数：3

共 16 条

[1] ACTION DES SELS DAMMONIUM QUATERNAIRE SUR LES ACIDES NUCLEIQUES .1. SOLUBILITE DES SELS DAMMONIUM QUATERNAIRE DES ACIDES NUCLEIQUES DANS LES SOLVANTS ORGANIQUES
AUBELSADRON, G
EBEL, JP
BECK, G
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1961, 53 (01) : 11 - &
[2] CHROMCZYNSKI P, 1991, CURRENT PROTOCOLS S, V14
[3] CYTOKINE GENE-EXPRESSION - ANALYSIS USING NORTHERN BLOTTING, POLYMERASE CHAIN-REACTION AND INSITU HYBRIDIZATION
DALLMAN, MJ
MONTGOMERY, RA
LARSEN, CP
WANDERS, A
WELLS, AF
[J]. IMMUNOLOGICAL REVIEWS, 1991, 119 : 163 - 179
[4] DELFAU MH, 1990, LEUKEMIA, V4, P1
[5] Hayakawa K., 1991, CATIONIC SURFACTANTS
[6] HIGUCHI R, 1989, PCR TECHNOLOGY
[7] THE ISOLATION OF BACTERIAL NUCLEIC ACIDS USING CETYLTRIMETHYLAMMONIUM BROMIDE (CETAVLON)
JONES, AS
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1953, 10 (04) : 607 - 612
[8] KAWASAKI ES, 1990, PCR PROTOCOLS GUIDE, P21
[9] KOWK S, 1992, PERKIN ELMER BIOTECH
[10] TWO-DIMENSIONAL BENZYLDIMETHYL-N-HEXADECYLAMMONIUM CHLORIDE-]SODIUM DODECYL-SULFATE PREPARATIVE POLYACRYLAMIDE-GEL ELECTROPHORESIS - A HIGH-CAPACITY HIGH-RESOLUTION TECHNIQUE FOR THE PURIFICATION OF PROTEINS FROM COMPLEX-MIXTURES
MACFARLANE, DE
[J]. ANALYTICAL BIOCHEMISTRY, 1989, 176 (02) : 457 - 463

← 1 2 →