NUCLEOTIDE-SEQUENCE ANALYSIS AND EPITOPE MAPPING OF THE MEROZOITE SURFACE PROTEIN-1 FROM PLASMODIUM-CHABAUDI CHABAUDI AS

被引：29

作者：

MCKEAN, PG ^{[1
]}

ODEA, K ^{[1
]}

BROWN, KN ^{[1
]}

机构：

[1] NATL INST MED RES,DIV PARASITOL,LONDON NW7 1AA,ENGLAND

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1993年 / 62卷 / 02期

关键词：

MALARIA; PLASMODIUM CHABAUDI CHABAUDI; MEROZOITE SURFACE PROTEIN 1; NUCLEOTIDE SEQUENCE ANALYSIS; EPITOPE MAPPING;

D O I：

10.1016/0166-6851(93)90109-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete nucleotide sequence of the gene encoding the merozoite surface protein 1 (MSP-1) from the rodent malaria parasite Plasmodium chabaudi chabaudi AS has been determined by direct sequencing of overlapping PCR derived fragments. Comparison of the P. c. chabaudi AS nucleotide sequence with the previously published P. c. chabaudi IP-PCI sequence indicates that although the MSP-1 gene of these two P. c. chabaudi strains is highly conserved, with sequence identity often approaching 100%, interspersed throughout the molecule are 5 regions of divergence. This is at variance with published data which suggested that the P. c. chabaudi AS and P. c. chabaudi IF-PCI MSP-1 sequences are largely identical. Epitope mapping studies with a panel of anti-P. c. chabaudi AS MSP-1 monoclonal antibodies demonstrate that whilst most of these mAbs recognise epitopes at the N-terminus of the MSP-1 molecule, two mAbs, including one capable of inhibiting challenge infections in mice in an in vivo passive transfer assay, recognise epitopes which map to the C-terminus.

引用

页码：199 / 209

页数：11

共 31 条

[1]

Hall, Osland, Hyde, Simmons, Hope, Scaife, Processing, polymorphism and biological significance of P190, a major surface antigen of the erythrocytic forms of Plasmodium falciparum, Mol. Biochem. Parasitol, 11, pp. 61-80, (1984)

[2]

Holder, Lockyer, Odink, Sandhu, Riveros-Moreno, Nicholls, Hillman, Davey, Tizard, Schwarz, Et al., Primary structure of the precursor to the three major surface antigens of Plasmodium falciparum merozoites, Nature, 317, pp. 270-273, (1985)

[3]

Boyle, Newbold, Smith, Brown, Monoclonal antibodies that protect in vivo against Plasmodium chabaudi recognise a 250 000- Dalton parasite polypeptide, Infect. Immun., 38, pp. 94-102, (1982)

[4]

Blackman, Heidrich, Donachie, McBride, Holder, A single fragment of a malaria merozoite surface protein remains on the parasite during red cell invasion is the target for invasion-inhibiting antibodies, J. Exp. Med., 172, pp. 379-382, (1990)

[5]

Holder, The precursor to major merozoite surface antigens: Structure and role in immunity, Prog. Allergy, 41, pp. 72-97, (1988)

[6]

Burns, Majarian, Young, Daly, Long, A protective monoclonal antibody recognises an epitope in the carboxyl-terminal cysteine-rich domain in the precursor of the major merozoite surface antigen of the rodent malarial parasitePlasmodium yoelii, J. Immunol., 143, pp. 2670-2676, (1989)

[7]

Mackay, Goman, Bone, Hyde, Scaife, Certa, Polymorphism of the precursor for the major surface antigens of Plasmodium falciparum merozoites: studies at the genetic level, EMBO J., 4, pp. 3823-3829, (1985)

[8]

Peterson, Coppel, McIntyre, Langford, Woodrow, Brown, Anders, Kemp, Variation in the precursor to the major merozoite surface antigens of Plasmodium falciparum, Mol. Biochem. Parasitol., 27, pp. 291-301, (1988)

[9]

Tanabe, Mackay, Goman, Scaife, Allelic dimorphism in a surface antigen gene of the malaria parasite Plasmodium falciparum, J. Mol. Biol., 195, pp. 273-287, (1987)

[10]

Kimura, Mattei, Mana di Santi, Scherf, Genetic diversity in the major merozoite surface antigen of Plasmodium falciparum: high prevalence of a third polymorphic form detected in strains derived from malaria patients, Gene, 91, pp. 57-62, (1990)

← 1 2 3 4 →