EFFECT OF CONJUGATION METHODOLOGY, CARRIER PROTEIN, AND ADJUVANTS ON THE IMMUNE-RESPONSE TO STAPHYLOCOCCUS-AUREUS CAPSULAR POLYSACCHARIDES

被引：39

作者：

FATTOM, A ^{[1
]}

LI, XR ^{[1
]}

CHO, YH ^{[1
]}

BURNS, A ^{[1
]}

HAWWARI, A ^{[1
]}

SHEPHERD, SE ^{[1
]}

COUGHLIN, R ^{[1
]}

WINSTON, S ^{[1
]}

NASO, R ^{[1
]}

机构：

[1] CAMBRIDGE BIOTECH CORP,CAMBRIDGE,MA

来源：

VACCINE | 1995年 / 13卷 / 14期

关键词：

ADJUVANTS; IGG SUBCLASSES; STAPHYLOCOCCUS CAPSULAR POLYSACCHARIDES; CONJUGATE VACCINES;

D O I：

10.1016/0264-410X(95)00052-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Conjugate vaccines were prepared with S. aureus type 8 capsular polysaccharide (CP) using three carrier proteins: Pseudomonas aeruginosa exotoxin A (ETA), a non-toxic recombinant ETA (rEPA), and diphtheria toroid (DTd). Adipic acid dihydrazide (ADH) or N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) was used as a spacer to link the CP to carrier protein All conjugates gave a high immune response with a boost after the second immunization. Conjugates prepared with ADH gave higher antibody titers than conjugates prepared with SPDP. IgG(1) was the primary subclass elicited by all conjugates regardless of the carrier protein or the conjugation method used to prepare the vaccines. The non-immunogenic CP and the conjugates were formulated with either monophosphoryl lipid A (MPL), QS21, or in Novasomes(TM) and evaluated in mice. While the adjuvants failed to improve the immunogenicity of the nonconjugated CP, a more than fivefold increase in the antibody levels was observed when these adjuvants were used with the conjugates. Significant rises in IgG(2b) and IgG(3) were observed with all formulations. The enhancement of the immunogenicity and the IgG subclass shift, as seen with some adjuvants, may prove to be important in immunocompromised patients.

引用

页码：1288 / 1293

页数：6

共 43 条

[1]

AUSTRIAN R, 1981, REV INFECT DIS, V3, pS51

[2] REGULATION OF MAGNITUDE OF ANTIBODY-RESPONSE TO BACTERIAL POLYSACCHARIDE ANTIGENS BY THYMUS-DERIVED LYMPHOCYTES [J].