IDENTIFICATION OF MITOTIC (CDC2) AND INTERPHASE HISTONE H1 KINASES BY NONDENATURING GEL-ELECTROPHORESIS AND PEPTIDE ASSAYS

被引：4

作者：

HOHMANN, P ^{[1
]}

GREENE, RS ^{[1
]}

机构：

[1] NIAGARA UNIV,DEPT BIOL,NIAGARA,NY 14109

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92387-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method of nondenaturing gel electrophoresis and a specific peptide based assay were used to study the histone kinases in mitotic and interphase mouse fibroblasts. The gels resolved four activities, one of which was shown to be the mitotic (CDC2) H1 kinase by virtue of its antigenicity. A new peptide substrate for the CDC2 kinase was phosphorylated by both S-phase and mitotic cell extracts and reacted with two protein kinases in the gels. Since the interphase enzyme did not react with the antibody, the results suggest that it is either a "masked" form of CDC2 or a second enzyme, functionally related to CDC2, which is responsible for the interphase phosphorylation of H1. © 1990.

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页码：763 / 770

页数：8

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