INVOLVEMENT OF RH BLOOD-GROUP POLYPEPTIDES IN THE MAINTENANCE OF AMINOPHOSPHOLIPID ASYMMETRY

被引：67

作者：

SCHROIT, AJ ^{[1
]}

BLOY, C ^{[1
]}

CONNOR, J ^{[1
]}

CARTRON, JP ^{[1
]}

机构：

[1] INST NATL TRANSFUSION SANGUINE,INSERM,U76,F-75015 PARIS,FRANCE

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 45期

关键词：

D O I：

10.1021/bi00497a003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, 125I-azido-PS, and with an inhibitor of PS transport, 125I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS. © 1990, American Chemical Society. All rights reserved.

引用

页码：10303 / 10306

页数：4

共 34 条

[1] BALLAS SK, 1984, BLOOD, V63, P1046
[2] BLANCHARD D, 1988, BLOOD, V72, P1424
[3] BLOY C, 1988, BLOOD, V72, P661
[4] CHARACTERIZATION OF THE D-ANTIGENS, C-ANTIGENS, E-ANTIGENS AND G-ANTIGENS OF THE RH BLOOD-GROUP SYSTEM WITH HUMAN MONOCLONAL-ANTIBODIES
BLOY, C
BLANCHARD, D
LAMBIN, P
GOOSSENS, D
ROUGER, P
SALMON, C
MASOUREDIS, SP
CARTRON, JP
[J]. MOLECULAR IMMUNOLOGY, 1988, 25 (09) : 925 - 930
[5] BLOY C, 1987, BLOOD, V69, P1491
[6] MOLECULAR-CLONING AND PROTEIN-STRUCTURE OF A HUMAN BLOOD-GROUP RH POLYPEPTIDE
CHERIFZAHAR, B
BLOY, C
LEVANKIM, C
BLANCHARD, D
BAILLY, P
HERMAND, P
SALMON, C
CARTRON, JP
COLIN, Y
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) : 6243 - 6247
[7] TRANSBILAYER MOVEMENT OF PHOSPHATIDYLSERINE IN NONHUMAN ERYTHROCYTES - EVIDENCE THAT THE AMINOPHOSPHOLIPID TRANSPORTER IS A UBIQUITOUS MEMBRANE-PROTEIN
CONNOR, J
SCHROIT, AJ
[J]. BIOCHEMISTRY, 1989, 28 (25) : 9680 - 9685
[8] TRANSBILAYER MOVEMENT OF PHOSPHATIDYLSERINE IN ERYTHROCYTES - INHIBITION OF TRANSPORT AND PREFERENTIAL LABELING OF A 31 000-DALTON PROTEIN BY SULFHYDRYL REACTIVE REAGENTS
CONNOR, J
SCHROIT, AJ
[J]. BIOCHEMISTRY, 1988, 27 (03) : 848 - 851
[9] AMINOPHOSPHOLIPID TRANSLOCATION IN ERYTHROCYTES - EVIDENCE FOR THE INVOLVEMENT OF A SPECIFIC TRANSPORTER AND AN ENDOFACIAL PROTEIN
CONNOR, J
SCHROIT, AJ
[J]. BIOCHEMISTRY, 1990, 29 (01) : 37 - 43
[10] INCORPORATION AND TRANSLOCATION OF AMINOPHOSPHOLIPIDS IN HUMAN-ERYTHROCYTES
DALEKE, DL
HUESTIS, WH
[J]. BIOCHEMISTRY, 1985, 24 (20) : 5406 - 5416

← 1 2 3 4 →