A SIMPLIFIED METHOD FOR SCREENING THE APOLIPOPROTEIN-E POLYMORPHISM

被引：11

作者：

EICHNER, JE ^{[1
]}

KULLER, LH ^{[1
]}

FERRELL, RE ^{[1
]}

KAMBOH, MI ^{[1
]}

机构：

[1] UNIV PITTSBURGH,GRAD SCH PUBL HLTH,DEPT HUMAN GENET,PITTSBURGH,PA 15261

来源：

HUMAN HEREDITY | 1991年 / 41卷 / 01期

关键词：

APOLIPOPROTEIN-E; POLYMORPHISMS; APO-E METHODS; APO-E SCREENING;

D O I：

10.1159/000153976

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A simple method for screening populations for the apolipoprotein E polymorphism which involves isoelectric focusing of delipidated samples on polyacrylamide gels of pH 4.5-5.8 followed by immunoblotting using a double-antibody technique is presented. The method is a synthesis of two previously published procedures and works well on samples that have been stored for as long as 15 years.

引用

页码：61 / 64

页数：4

共 12 条

[1] THE RECEPTOR MODEL FOR TRANSPORT OF CHOLESTEROL IN PLASMA
BROWN, MS
GOLDSTEIN, JL
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1985, 454 : 178 - 182
[2] APOLIPOPROTEIN-E POLYMORPHISM AND ATHEROSCLEROSIS
DAVIGNON, J
GREGG, RE
SING, CF
[J]. ARTERIOSCLEROSIS, 1988, 8 (01): : 1 - 21
[3] PHENOTYPIC EFFECTS OF APOLIPOPROTEIN STRUCTURAL VARIATION ON LIPID PROFILES .3. CONTRIBUTION OF APOLIPOPROTEIN-E PHENOTYPE TO PREDICTION OF TOTAL CHOLESTEROL, APOLIPOPROTEIN-B, AND LOW-DENSITY-LIPOPROTEIN CHOLESTEROL IN THE HEALTHY WOMEN STUDY
EICHNER, JE
KULLER, LH
FERRELL, RE
MEILAHN, EN
KAMBOH, MI
[J]. ARTERIOSCLEROSIS, 1990, 10 (03): : 379 - 385
[4] PHENOTYPIC EFFECTS OF APOLIPOPROTEIN STRUCTURAL VARIATION ON LIPID PROFILES .4. APOLIPOPROTEIN POLYMORPHISMS IN A SMALL-GROUP OF BLACK-WOMEN FROM THE HEALTHY WOMEN STUDY
EICHNER, JE
KULLER, LH
FERRELL, RE
KAMBOH, MI
[J]. GENETIC EPIDEMIOLOGY, 1989, 6 (06) : 681 - 689
[5] ABNORMAL INVIVO METABOLISM OF APOLIPOPROTEIN-E4 IN HUMANS
GREGG, RE
ZECH, LA
SCHAEFER, EJ
STARK, D
WILSON, D
BREWER, HB
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1986, 78 (03) : 815 - 821
[6] GREGG RE, 1984, J LIPID RES, V25, P1167
[7] HAVEKES LM, 1987, J LIPID RES, V28, P455
[8] KAMBOH MI, 1988, J LIPID RES, V29, P1535
[9] LIPOPROTEIN RECEPTORS AND CHOLESTEROL HOMEOSTASIS
MAHLEY, RW
INNERARITY, TL
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 737 (02) : 197 - 222
[10] EDUCATIONAL-ATTAINMENT AND BEHAVIORAL AND BIOLOGIC RISK-FACTORS FOR CORONARY HEART-DISEASE IN MIDDLE-AGED WOMEN
MATTHEWS, KA
KELSEY, SF
MEILAHN, EN
KULLER, LH
WING, RR
[J]. AMERICAN JOURNAL OF EPIDEMIOLOGY, 1989, 129 (06) : 1132 - 1144

← 1 2 →