TEMPERATURE CONTROLS BOTH GAMETOPHYTIC AND SPOROPHYTIC DEVELOPMENT IN MICROSPORE CULTURES OF BRASSICA-NAPUS

被引:137
作者
CUSTERS, JBM
CORDEWENER, JHG
NOLLEN, Y
DONS, HJM
CAMPAGNE, MMV
机构
[1] Department of Developmental Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), AA Wageningen, 6700
关键词
POLLEN MATURATION; EMBRYOGENESIS; RAPESEED; ANDROGENESIS; HAPLOID PLANT;
D O I
10.1007/BF00233317
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Temperature controls the developmental fate of isolated Brassica napus microspores in vitro. Culture at 32.5 degrees C leads to sporophytic development and the formation of embryos. Here we show that culture at 17.5 degrees C leads to gametophytic development, and the formation of pollen-like structures at high frequencies (up to 80% after 7 days in culture). Early stages of both developmental pathways are observed in culture at 25.0 degrees C, and embryos are produced at low frequencies (0.7%) at that temperature. Culturing B. napus microspores at 32.5 degrees C versus 17.5 degrees C brings the switch from gametophytic to sporophytic development under simple experimental control and provides a convenient tool for investigating the cellular and molecular mechanisms controlling this developmental switch.
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页码:267 / 271
页数:5
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