EXPRESSION OF 3 OSMOTIN-LIKE PROTEIN GENES IN RESPONSE TO OSMOTIC-STRESS AND FUNGAL INFECTION IN POTATO

被引:71
作者
ZHU, BL
CHEN, THH
LI, PH
机构
[1] UNIV MINNESOTA,DEPT HORT SCI,PLANT HARDINESS LAB,ST PAUL,MN 55108
[2] UNIV MINNESOTA,PLANT BIOL SCI PROGRAM,ST PAUL,MN 55108
[3] OREGON STATE UNIV,CTR GENE RES & BIOTECHNOL,DEPT HORT,CORVALLIS,OR 97331
关键词
OSMOTIC-STRESS; OSMOTIN-LIKE PROTEINS; PATHOGENESIS-RELATED PROTEINS; PHYTOPHTHORA INFESTANS; SALICYLIC ACID; STRESS-INDUCIBLE GENES;
D O I
10.1007/BF00042034
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have characterized three cDNAs encoding osmotin-like proteins from potato (Solanum commersonii) cell cultures. These cDNAs (pA13, pA35, and pA81) have extensive nucleotide identity in the coding regions but low homology in the 3' non-coding sequences, and may encode three isoforms of potato pathogenesis-related (PR) type 5 proteins. Using gene-specific probes, RNA gel blot analyses showed constitutive accumulation of osmotin-like protein mRNAs in cell cultures, leaves, stems, roots and flowers, with high abundance in the roots and mature flowers. Treatments with abscisic acid (ABA), low temperature, and NaCl increased the accumulation of all three mRNAs in S. commersonii cell cultures and plants grown in vitro. Salicylic acid (SA), and wounding resulted in a moderate increase in the levels of pA13 and pA81 but not pA35 mRNAs. Infection with the fungus Phytophthora infestans activated strong and non-systemic expression of all three osmotin-like protein genes. The accumulation of osmotin-like proteins, however, was detected only in P. infestans-infected tissues but not in plants treated with ABA, SA, NaCl, low temperature, or wounding.
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页码:17 / 26
页数:10
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