METHOD FOR ULTRASTRUCTURAL STUDIES OF THE INTACT TISSUE-METAL INTERFACE

被引：53

作者：

BJURSTEN, LM

EMANUELSSON, L

ERICSON, LE

THOMSEN, P

LAUSMAA, J

MATTSSON, L

ROLANDER, U

KASEMO, B

机构：

[1] GOTHENBURG UNIV,DEPT ANAT,S-40033 GOTHENBURG,SWEDEN

[2] CHALMERS UNIV TECHNOL,DEPT PHYS,S-41296 GOTHENBURG,SWEDEN

来源：

BIOMATERIALS | 1990年 / 11卷 / 08期

关键词：

cell-material interface; Titanium; ultrastructure;

D O I：

10.1016/0142-9612(90)90085-5

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Samples were prepared for ultrastructural studies of the intact interface between metallic implants and tissue by transmission electron microscopy. The method is based on plastic embedding of implant and tissue and subsequent removal of the bulk metal by electrochemical dissolution (electropolishing), to facilitate preparation of ultrathin sections for transmission electron microscopy. Surface sensitive spectroscopy (Auger electron microscopy and X-ray photoemission spectroscopy) and transmission electron microscopy EDX results show that the method produces samples with an intact interface, containing the implant surface oxide and the adjacent tissue. Examples of application of the method on titanium, zirconium and aluminium implants in soft tissue are given. © 1990.

引用

页码：596 / 601

页数：6

共 14 条

[1]

ALBREKTSSON T, 1982, ADV BIOMATERIALS CLI, V4, P166

[2] MICROSTRUCTURE AND MORPHOLOGY OF SURFACE OXIDE-FILMS ON TI-6AL-4V [J].