NEW METHOD FOR SCANNING ELECTRON-MICROSCOPIC VISUALIZATION OF DERMAL ELASTIC FIBERS

被引:51
作者
TSUJI, T [1 ]
LAVKER, RM [1 ]
KLIGMAN, AM [1 ]
机构
[1] UNIV PENN, DUHRING LABS, DEPT DERMATOL, PHILADELPHIA, PA 19104 USA
关键词
D O I
10.1111/j.1365-2818.1979.tb00166.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
Autoclaving sections of dermis for 8 h followed by fixation, dehydration and xylol‐air drying yields a pure preparation of elastic fibres for scanning electron microscopy which retains the native architecture of this component. Elastic fibres were intertwined in a complex fashion with numerous branches. Fibres were predominantly cylindrical in the upper dermis and became larger and more elliptical in the deeper dermis. This method produces a means to study the organization of elastic fibres in a variety of disorders in which dermal changes are prominent. 1979 Blackwell Science Ltd
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页码:165 / 173
页数:9
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