AGROBACTERIUM - GENETIC-STUDIES ON AGROCIN-84 PRODUCTION AND THE BIOLOGICAL-CONTROL OF CROWN GALL

被引:78
作者
ELLIS, JG [1 ]
KERR, A [1 ]
VANMONTAGU, M [1 ]
SCHELL, J [1 ]
机构
[1] STATE UNIV GHENT,B-9000 GHENT,BELGIUM
来源
PHYSIOLOGICAL PLANT PATHOLOGY | 1979年 / 15卷 / 03期
关键词
D O I
10.1016/0048-4059(79)90082-1
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The rôle of the bacteriocin, agrocin 84, in the biological control. of crown gall was investigated by constructing new bacteriocinogenic strains. A small plasmid which codes for the ability to produce agrocin 84 has been identified and transferred to recipient strains by conjugation. Two donor strains were used; the avirulent strain 84 and the virulent strain 398. Agrocin production and virulence in the latter strain have been separated by genetic manipulation and avirulent, agrocin producing derivatives have been produced. Avirulent transconjugants which produced agrocin 84 varied in their effectiveness as biological control gents. Evidence is presented that this was probably due to variation in rate of growth of the different strains in the inoculation sites. © 1979.
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页码:311 / &
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共 30 条
  • [1] A BIOCHEMICAL TEST FOR CROWN GALL BACTERIA
    BERNAERTS, MJ
    DELEY, J
    [J]. NATURE, 1963, 197 (486) : 406 - &
  • [2] BOMHOFF G, 1976, MOL GEN GENET, V145, P171
  • [3] CHILTON MD, 1976, GENETICS, V83, P609
  • [4] ISOLATION OF COVALENTLY CLOSED CIRCULAR DNA OF HIGH MOLECULAR-WEIGHT FROM BACTERIA
    CURRIER, TC
    NESTER, EW
    [J]. ANALYTICAL BIOCHEMISTRY, 1976, 76 (02) : 431 - 441
  • [5] DHANVANTARI BN, 1976, PLANT DIS REP, V60, P549
  • [6] ELLIS JG, 1979, SOIL BORNE PATHOGENS
  • [7] AGROCIN-84 SENSITIVITY - PLASMID DETERMINED PROPERTY IN AGROBACTERIUM-TUMEFACIENS
    ENGLER, G
    HOLSTERS, M
    VANMONTAGU, M
    SCHELL, J
    HERNALSTEENS, JP
    SCHILPEROORT, R
    [J]. MOLECULAR & GENERAL GENETICS, 1975, 138 (04): : 345 - 349
  • [8] TI PLASMIDS OF AGROBACTERIUM AS CONJUGATIVE PLASMIDS
    GENETELLO, C
    VANLAREBEKE, N
    HOLSTERS, M
    DEPICKER, A
    VANMONTAGU, M
    SCHELL, J
    [J]. NATURE, 1977, 265 (5594) : 561 - 563
  • [9] CHROMOSOME MOBILIZATION BY R PLASMID R68.45 - TOOL IN PSEUDOMONAS GENETICS
    HAAS, D
    HOLLOWAY, BW
    [J]. MOLECULAR & GENERAL GENETICS, 1978, 158 (03): : 229 - 237
  • [10] ISOLATION OF LARGE BACTERIAL PLASMIDS AND CHARACTERIZATION OF P2 I COMPATIBILITY GROUP PLASMIDS PMG1 AND PMG5
    HANSEN, JB
    OLSEN, RH
    [J]. JOURNAL OF BACTERIOLOGY, 1978, 135 (01) : 227 - 238