CLONING OF A CDNA-ENCODING AN ARTEMIA-FRANCISCANA NA/K ATPASE ALPHA-SUBUNIT

被引：28

作者：

MACIAS, MT

PALMERO, I

SASTRE, L

机构：

[1] CSIC, INST INVEST BIOMED, C ARTURO DUPERIER 4, E-28029 MADRID, SPAIN

[2] UNIV AUTONOMA MADRID, DEPT BIOQUIM, E-28029 MADRID, SPAIN

来源：

GENE | 1991年 / 105卷 / 02期

关键词：

BRINE SHRIMP; CRUSTACEAN; RECOMBINANT DNA; CONSERVED DOMAINS; GENE EXPRESSION; EVOLUTION; SEQUENCING;

D O I：

10.1016/0378-1119(91)90151-Z

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Clones of cDNA that code for an isoform of the Artemia franciscana Na/K ATPase-alpha subunit (NaKA-alpha) have been isolated. The sequence of the longest of these clones (pArATNa136) is 3595 nucleotides; it codes for a 1004-amino acid protein whose Sequence is identical to that of two previously sequenced Artemia NaKA-alpha peptides. The encoded protein is over 73% identical to Drosophila melanogaster and vertebrate NaKA-alphas, and 73.8% identical to another Artemia NaKA-alpha isoform previously described (named alpha-2850 in this article). The two Artemia cDNA clones code for mRNAs of different size; the clone pArATNa136 codes for a 4.5-kb mRNA while the alpha-2850 clone codes for a 3.6-kb mRNA. The degree of homology and the different size of the mRNAs encoded by both cDNAs suggest that they code for two different isoforms of the protein.

引用

页码：197 / 204

页数：8

共 42 条

[1] LEVELS OF TRANSLATABLE MESSENGER-RNAS FOR CELL-SURFACE PROTEIN, COLLAGEN PRECURSORS, AND 2 MEMBRANE PROTEINS ARE ALTERED IN ROUS-SARCOMA VIRUS-TRANSFORMED CHICK-EMBRYO FIBROBLASTS [J].