LECTIN-BINDING STUDIES WITH FITC-MARKED WGA AND UEA-I AND FLOW-CYTOMETRIC MEASUREMENTS ON ISOLATED RAT PANCREATIC ACINAR-CELLS

被引：5

作者：

JONAS, L ^{[1
]}

FULDA, G ^{[1
]}

WALZEL, H ^{[1
]}

SCHULZ, U ^{[1
]}

机构：

[1] UNIV ROSTOCK,FAC MED,DEPT IMMUNOL,D-18057 ROSTOCK,GERMANY

来源：

ACTA HISTOCHEMICA | 1993年 / 95卷 / 01期

关键词：

RAT PANCREAS; ACINAR CELLS; COLLAGENASE DIGESTION; LECTIN BINDING; FLUORESCENCE; FLOW-CYTOMETRIC;

D O I：

10.1016/S0065-1281(11)80386-7

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Lectin binding to the glycocalyx of isolated rat pancreatic acinar cells was studied by flowcytometric measurements. The pancreatic exocrine cells were prepared after collagenase digestion and cleaned in a density gradient. The fluorescence of cells was measured in a FACScan after binding of FITC marked WGA or UEA I. The binding of lectins was inhibited by preabsorption of WGA-FITC with N-acetyl-glucosamin, sialic acid or chitinoses and by preabsorption of UEA-FITC with alpha-L-fucose, respectively. Furthermore, we were able to measure a decreased WGA-FITC and UEA-FITC binding after a short preincubation of isolated cells with the peptide hormone cholecystokinin and its agonists (caerulein, pentagastrin).

引用

页码：45 / 52

页数：8

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