PAROMOMYCIN RESISTANCE IN LEISHMANIA-TROPICA - LACK OF CORRELATION WITH MUTATION IN THE SMALL-SUBUNIT RIBOSOMAL-RNA GENE

被引:34
作者
FONG, D
CHAN, MMY
RODRIGUEZ, R
GATELY, LJ
BERMAN, JD
GROGL, M
机构
[1] RUTGERS STATE UNIV, BUR BIOL RES, PISCATAWAY, NJ 08855 USA
[2] WALTER REED ARMY MED CTR, WALTER REED ARMY INST RES, DIV EXPTL THERAPEUT, WASHINGTON, DC 20307 USA
关键词
D O I
10.4269/ajtmh.1994.51.758
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
The aminoglycoside antibiotic paromomycin is a potentially useful anti-leishmanial chemotherapeutic agent. Resistance to this antibiotic was studied using Leishmania tropica. Promastigotes resistant to 210 mu g/ml of paromomycin were selected by exposing them to gradual increments of this drug. Previous work in Escherichia coli, Tetrahymena, and yeast mitochondrial mutants has demonstrated mutations in the E. coli small subunit ribosomal RNA at the 1409:1491 basepair position, or equivalent positions in other organisms, resulting in basepair disruption. When the nucleotide sequence at both the DNA and RNA levels of the resistant L. tropica promastigotes cultured in the presence of paromomycin was compared with those of the drug-sensitive parent, there was no sequence change at the putative mutation site. Paromomycin resistance in L. tropica is apparently due to other mechanisms.
引用
收藏
页码:758 / 766
页数:9
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