BROMELIN TREATMENT OF RED BLOOD CELLS

被引：4

作者：

MILNER, LV

机构：

[1] The Natal Blood Transfusion Service, Durban

来源：

TRANSFUSION | 1968年 / 8卷 / 01期

关键词：

D O I：

10.1111/j.1537-2995.1968.tb02386.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The data presented shows that the most effective method for the bromelinization of red blood cells when using 0.5% bromelin, is to incubate equal volumes of an 80% suspension of cells in saline with an equal volume of bromelin at 37 C for 15 minutes. Forty per cent of antibodies will give better reactions at some other suspension of red cells and 25% require a different incubation time. Fifteen minutes is the optimum incubation time for the direct test, but 55% of the antibodies tested in this series had a different incubation period. The indirect test gave titers equal to or higher than the direct test with 86.6% of the antibodies tested. It gave titers one dilution higher in 47.2%, two dilutions higher in 8.33% and 3 tubes higher in 2.78% of the antibodies tested. 1968 AABB

引用

页码：39 / &