PREPARATION OF HIGHLY PURIFIED 3ALPHA-HYDROXYSTEROID AND 3BETA-HYDROXYSTEROID DEHYDROGENASES FROM PSEUDOMONAS-SP

被引：21

作者：

SHIKITA, M ^{[1
]}

TALALAY, P ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT,BALTIMORE,MD 21205

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90218-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for preparing highly purified 3α- and 3β-hydroxysteroid dehydrogenases (EC 1.1.1.50 and EC 1.1.1.145, respectively), essentially uncontaminated with one another, from extracts of a steroid-induced Pseudomonas species. These enzymes are suitable for the microanalysis of 3α-hydroxy-, 3β-hydroxy-, and 3-ketosteroids. © 1979.

引用

页码：286 / 292

页数：7

共 31 条

[1]

AUGRUST LE, 1976, BIOCH BIOPHYS ACTA, V438, P13

[2]

BATTAIS E, 1977, BIOCHIMIE, V59, P909

[3]

Benson A M, 1974, Methods Enzymol, V34, P557

[4] PURIFICATION AND PROPERTIES OF A 3ALPHA-HYDROXYSTEROID DEHYDROGENASE FROM PSEUDOMONAS TESTOSTERONI [J].

BOYER, J ;

BARON, DN ;

TALALAY, P .

BIOCHEMISTRY, 1965, 4 (09) :1825-&

[5] QUANTITATIVE DETERMINATION OF MAJOR 3-HYDROXY BILE ACIDS IN BIOLOGICAL MATERIAL AFTER THIN-LAYER CHROMATOGRAPHIC SEPARATION [J].

BRUUSGAA.A .

CLINICA CHIMICA ACTA, 1970, 28 (03) :495-&

[6] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].

DAVIS, BJ .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) :404-&

[7] PURIFICATION OF STEROID-INDUCED ENZYMES FROM PSEUDOMONAS TESTOSTERONI [J].

DELIN, S ;

SQUIRE, PG ;

PORATH, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 89 (03) :398-+

[8]

DELIN S, 1963, BIOCHIM BIOPHYS ACTA, V67, P197

[9] HYDROXYSTEROID DEHYDROGENASES OF PSEUDOMONAS-TESTOSTERONI - SEPARATION OF A 17BETA-HYDROXYSTEROID DEHYDROGENASE FROM 3(17)BETA-HYDROXYSTEROID DEHYDROGENASE AND COMPARISON OF 2 ENZYMES [J].

GROMAN, EV ;

ENGEL, LL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 485 (02) :249-254

[10] ENZYMATIC TECHNIQUES IN STEROID ASSAY [J].

HARKONEN, M ;

ADLERCREUTZ, H ;

GROMAN, EV .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1974, 5 (08) :717-725

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