SIMPLIFIED ASSAY OF AMRINONE IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：6

作者：

LAWLESS, S

BURCKART, G

PICCOLA, G

DIVEN, W

MILES, M

ZARITSKY, A

机构：

[1] UNIV N CAROLINA,DEPT PEDIAT,CHAPEL HILL,NC 27514

[2] UNIV N CAROLINA,DEPT PHARM PRACTICE,CHAPEL HILL,NC 27514

[3] UNIV PITTSBURGH,DEPT PHARMACOL,PITTSBURGH,PA 15260

[4] UNIV PITTSBURGH,DEPT CHEM,PITTSBURGH,PA 15260

来源：

THERAPEUTIC DRUG MONITORING | 1990年 / 12卷 / 06期

关键词：

Amrinone; Assay; High-performance liquid chromatography;

D O I：

10.1097/00007691-199011000-00010

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

An isocratic high-performance liquid chromatography method for the assay of plasma amrinone is described. Plasma amrinone is extracted using protein precipitation with an internal standard, separated with a reverse phase column, and detected using ultraviolet absorption. Each run is completed within 10 min. The assay can detect amrinone concentrations between 0.5 and 10.0 μg/ml, within the accepted therapeutic range. The assay has a within-day coefficient of variation of 5% and a day-to-day coefficient of variation of 10% in the therapeutic range of amrinone. This technique is an accurate, simple, and rapid method for the determination of amrinone concentrations in plasma. © 1990 Raven Press, Ltd.

引用

收藏

页码：570 / 573

页数：4

相关论文

共 12 条

[1] DEVELOPMENTAL-CHANGES IN THE CARDIAC EFFECTS OF AMRINONE IN THE DOG [J].

BINAH, O ;

LEGATO, MJ ;

DANILO, P ;

ROSEN, MR .

CIRCULATION RESEARCH, 1983, 52 (06) :747-752

[2] HEMODYNAMIC CONSEQUENCES OF INOTROPIC SUPPORT WITH DIGOXIN OR AMRINONE IN LAMBS WITH VENTRICULAR SEPTAL-DEFECT [J].

BOUCEK, MM ;

CHANG, R ;

SYNHORST, DP .

PEDIATRIC RESEARCH, 1985, 19 (09) :887-891

[3] RELATIONSHIP BETWEEN AMRINONE PLASMA-CONCENTRATION AND CARDIAC INDEX [J].

EDELSON, J ;

LEJEMTEL, TH ;

ALOUSI, AA ;

BIDDLECOME, CE ;

MASKIN, CS ;

SONNENBLICK, EH .

CLINICAL PHARMACOLOGY & THERAPEUTICS, 1981, 29 (06) :723-728

[4] PRELIMINARY-RESULTS WITH AMRINONE IN PERIOPERATIVE LOW CARDIAC-OUTPUT SYNDROME [J].

GUNNICKER, M ;

HESS, W .

THORACIC AND CARDIOVASCULAR SURGEON, 1987, 35 (04) :219-225

[5] EFFECT OF AMRINONE ON RIGHT VENTRICULAR-FUNCTION - PREDOMINANCE OF AFTERLOAD REDUCTION [J].

KONSTAM, MA ;

COHEN, SR ;

SALEM, DN ;

DAS, D ;

ARONOVITZ, MJ ;

BROCKWAY, BA .

CIRCULATION, 1986, 74 (02) :359-366

[6] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMRINONE AND ITS N-ACETYL DERIVATIVE IN PLASMA - PHARMACOKINETICS OF AMRINONE IN THE DOG [J].

KULLBERG, MP ;

DORRBECKER, B ;

LENNON, J ;

ROWE, E ;

EDELSON, J .

JOURNAL OF CHROMATOGRAPHY, 1980, 187 (01) :264-270

[7] AMRINONE IN NEONATES AND INFANTS AFTER CARDIAC-SURGERY [J].

LAWLESS, S ;

BURCKART, G ;

DIVEN, W ;

THOMPSON, A ;

SIEWERS, R .

CRITICAL CARE MEDICINE, 1989, 17 (08) :751-754

[8] INTRAVENOUS USE OF AMRINONE FOR THE TREATMENT OF THE FAILING HEART [J].

MANCINI, D ;

LEJEMTEL, T ;

SONNENBLICK, E .

AMERICAN JOURNAL OF CARDIOLOGY, 1985, 56 (03) :B8-B15

[9] COMBINATION HIGH-DOSE AMRINONE AND DOPAMINE IN THE MANAGEMENT OF MORIBUND CARDIOGENIC-SHOCK AFTER OPEN-HEART SURGERY [J].

OLSEN, KH ;

KLUGER, J ;

FIELDMAN, A .

CHEST, 1988, 94 (03) :503-506

[10] NEGATIVE INOTROPIC EFFECTS OF AMRINONE IN THE NEONATAL PIGLET HEART [J].

ROSSASCUITTO, N ;

ASCUITTO, R ;

CHEN, V ;

DOWNING, SE .

CIRCULATION RESEARCH, 1987, 61 (06) :847-852