REQUIREMENT FOR ACTIVIN-A AND TRANSFORMING GROWTH FACTOR-BETA-1 PRO-REGIONS IN HOMODIMER ASSEMBLY

被引：240

作者：

GRAY, AM ^{[1
]}

MASON, AJ ^{[1
]}

机构：

[1] GENENTECH INC,DEPT DEV BIOL,S SAN FRANCISCO,CA 94080

来源：

SCIENCE | 1990年 / 247卷 / 4948期

关键词：

D O I：

10.1126/science.2315700

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Many proteins are initially synthesized as part of a large precursor. The role of the proregion in the biosynthesis of transforming growth factor-β1 (TGF-β1) and activin A, two structurally related disulfide-linked homodimers synthesized as large precursors, was studied. Vectors that expressed either the pro-region or the mature regions of these molecules were used in complementation experiments, only when the pro-region was coexpressed with the mature region did intracellular dimerization and secretion of biologically active homodimers occur. The pro-regions of activin A and TGF-β1, therefore, aid the folding, disulfide bond formation, and export of their respective homodimers.

引用

页码：1328 / 1330

页数：3

共 15 条

[1] HUMAN TRANSFORMING GROWTH FACTOR-BETA COMPLEMENTARY-DNA SEQUENCE AND EXPRESSION IN NORMAL AND TRANSFORMED-CELLS [J].