EVIDENCE FOR THE RESISTANCE OF THYROTROPIN-RELEASING-HORMONE (TRH) AND PSEUDO-HORMONE, PYROGLUTAMYL-HISTIDYL-AMPHETAMINE, TO DEGRADATION BY ENZYMES OF THE DIGESTIVE-TRACT INVITRO

TRH and pseudo-hormone (pyro Glu-Hisamphetamine) were submitted to the digestion of chymotrypsin and prolidase and independently to the digestion of enzymes of the digestive track: pepsin (stomach), pancreatins (pancreas) and enzymes extracted from the intestinal mucosa (small intestine). Using thin layer chromatography and high voltage electrophoresis techniques to detect enzymic digestion products, only intact TRH and pseudo-hormone were found, indicating that both entities were, under the conditions used, resistant to in vitro digestion by enzymes of the digestive tract. © 1979 Masson, Paris.