ONTOGENY OF THE F8FAMIDE-LIKE (MORPHINE-MODULATING) PEPTIDES IN THE CENTRAL-NERVOUS-SYSTEM OF RATS

FLFQPQRF-NH2 (F8Famide; FMRFamide-like peptide; morphine-modulating peptide) is a peptide isolated from bovine brain. It has some opiate analgesia modulating effects. In an adult rat central nervous system, F8Famide-like immunoreactivity is found in high concentrations in the posterior pituitary, hypothalamus, pons, medulla, and dorsal spinal cord. By using immunohistochemistry and radioimmunoassay, we studied the development of the F8Famide-immunoreactive system in the rat central nervous system during the ontogeny. F8Famide-immoreactive fibers and terminals first appeared in the median eminence on the twentieth embryonal day. Postnatally the F8Famide-immunoreactive system developed rapidly both in the brain and spinal cord, the immunoreactive structures having an adultlike distribution by the age of 1 week. A transient increase of the F8Famide-immunoreactive material was observed during the third and fourth postnatal weeks. By the age of 4 weeks, the F8Famide-containing neuronal system was adultlike both in distribution and quantity. The results suggest that the F8Famide-like peptides may act as neurotransmitters or neuromodulators from the time of their appearance in the brain and spinal cord. The early appearance and the distribution of the F8Famide-like immunoreactivity suggest that these peptides may participate, in addition to nociceptive mechanisms, in the regulation of blood pressure, feeding behaviour, and endocrine functions.