AFFINITY PURIFICATION OF ANTIBODIES USING IMMOBILIZED FB DOMAIN OF PROTEIN-A

被引：6

作者：

SOLOMON, B

RAVIV, O

LEIBMAN, E

FLEMINGER, G

机构：

[1] Department of Molecular Microbiology and Biotechnology, George Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 597卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80118-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A continuous method for the efficient digestion of protein A into active fragments (FB, M(r) = 7000) using immobilized trypsin was developed. These fragments originate from almost identical five-repeated monovalent Fc-binding units of 58 residues each. The fragments obtained were found to be similar to the recently described genetically engineered fragment B. Antibody-binding characteristics of the FB domain and also of intact protein A, immobilized on to adipic dihydrazide-modified Eupergit CB6200 beads, were investigated. Based on the experimental data obtained, a high-performance liquid chromatographic column containing C30N Eupergit C-immobilized FB domain was prepared and its performance in antibody purification was compared with that of Eupergit C-immobilized intact protein A.

引用

页码：257 / 262

页数：6

共 13 条

[1] CRYSTALLOGRAPHIC REFINEMENT AND ATOMIC MODELS OF A HUMAN FC FRAGMENT AND ITS COMPLEX WITH FRAGMENT-B OF PROTEIN-A FROM STAPHYLOCOCCUS-AUREUS AT 2.9-A AND 2.8-A RESOLUTION [J].

DEISENHOFER, J .

BIOCHEMISTRY, 1981, 20 (09) :2361-2370

[2] EFFECT OF POLYETHYLENE-GLYCOL ON THE NONSPECIFIC ADSORPTION OF PROTEINS TO EUPERGIT-C AND AGAROSE [J].

FLEMINGER, G ;

SOLOMON, B ;

WOLF, T ;

HADAS, E .

JOURNAL OF CHROMATOGRAPHY, 1990, 510 :271-279

[3] COMPARISON OF IMMUNOGLOBULIN BINDING-CAPACITIES AND LIGAND LEAKAGE USING 8 DIFFERENT PROTEIN-A AFFINITY-CHROMATOGRAPHY MATRICES [J].

FUGLISTALLER, P .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 124 (02) :171-177

[4]

HJELM H, 1975, EUR J BIOCHEM, V57, P395, DOI 10.1111/j.1432-1033.1975.tb02313.x

[5] PROTEIN A FROM STAPHYLOCOCCUS-AUREUS - ITS ISOLATION BY AFFINITY CHROMATOGRAPHY AND ITS USE AS AN IMMUNOSORBENT FOR ISOLATION OF IMMUNOGLOBULINS [J].

HJELM, H ;

HJELM, K ;

SJOQUIST, J .

FEBS LETTERS, 1972, 28 (01) :73-&

[6]

HOU KC, 1991, BIOTECHNOL APPL BIOC, V13, P257

[7] APPLICATIONS OF IMMOBILIZED PROTEIN-A IN IMMUNOCHEMICAL TECHNIQUES [J].

LANGONE, JJ .

JOURNAL OF IMMUNOLOGICAL METHODS, 1982, 55 (03) :277-296

[8]

NGO TT, 9TH INT S AFF CHROM

[9] HIGH-LEVEL EXPRESSION OF A SYNTHETIC GENE CODING FOR IGG-BINDING DOMAIN-B OF STAPHYLOCOCCAL PROTEIN-A [J].

SAITO, A ;

HONDA, S ;

NISHI, T ;

KOIKE, M ;

OKAZAKI, K ;

ITOH, S ;

SATO, M .

PROTEIN ENGINEERING, 1989, 2 (06) :481-487

[10] THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS [J].

SCATCHARD, G .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1949, 51 (04) :660-672

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