ENZYMURIA AND TUBULAR PROTEINURIA IN DIABETIC RATS - A 12-WEEK FOLLOW-UP-STUDY

被引：5

作者：

CHOUINARD, S ^{[1
]}

VIAU, C ^{[1
]}

GRESELIN, E ^{[1
]}

机构：

[1] UNIV MONTREAL,FAC MED,DEPT MED TRAVAIL & HYG MILIEU,CP 6128,SUCC A,MONTREAL H3C 3J7,QUEBEC,CANADA

来源：

RENAL FAILURE | 1992年 / 14卷 / 01期

基金：

英国医学研究理事会;

关键词：

DIABETIC NEPHROPATHY; ENZYMURIA; BETA-2-MICROGLOBULIN; PROTEINURIA; RAT; RENAL TUBULES; STREPTOZOTOCIN;

D O I：

10.3109/08860229209039115

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Various biochemical parameters of renal tubular function were examined for a period of up to 12 weeks in rats rendered diabetic by an i.v. injection of streptozotocin. Except for a statistically significant decrease in the urinary excretion of gamma-glutamyl-transpeptidase to 64% of control values, the urinary excretion of beta-N-acetyl-D-glucosaminidase, beta-galactosidase, alanine aminopeptidase, and lactate dehydrogenase significantly increases in diabetic rats to between 154% and 712% of control values. This increased enzymuria is not correlated to the marked polyuria induced by diabetes (r between 0.14 and 0.35, not significant). Enzymuria is also accompanied by a 10-fold increase in the urinary excretion of the low molecular weight protein beta(2)-microglobulin while the excretion of albumin is not significantly modified, indicating impairment of tubular reabsorption in diabetic animals. Clearance studies reveal that the clearance of both beta(2)-microglobulin and infused egg-white lysozyme are also increased. Finally the histopathologic examination of paraffin sections of the kidney show hydropic degenerescence and pycnosis of the tubular cells. It is concluded that early-stage diabetes results in tubular impairment and that the streptozotocin-rat model appears well suited to the study of these early signs of renal dysfunction.

引用

页码：41 / 47

页数：7

共 28 条

[1]

Deckert T., Parving H.-H., Andersen A.R., Christiansen J.S., Oxen-Boll B., Svendsen P.A.a., Telmer S., Christy M., Lauritzen T., Thomsen O.F., Kreiner S., Anderson J.R., Binder C., Diabetic nephropathy: A clinical and morphometric study, Advances in Diabetes Epidemiology, pp. 235-243, (1982)

[2]

Andersen A.R., Christiansen J.S., Andersen J.K., Kreiner S., Deckert T., Diabetic nephropathy in type I (insulin-dependent) diabetes: An epidemological study, Diabetologia, 25, pp. 493-501, (1983)

[3]

Oakley W.G., Pyke D.A., Tattersall R.B., Watkins P.J., Long-term diabetes: A clinical study of 92 patients after 40 years, Q J Med., 43, pp. 146-156, (1974)

[4]

Mathiesen E.R., Oxenboll B., Johansen P.A.a., Sevendsen P.A., Deckert T., Incipient nephropathy in type I (insulin-dependent) diabetes, Diabetologia, 26, pp. 406-410, (1984)

[5]

Viberti G.C., Jarrett R.J., Mahmud U., Hill R.P., Argyropoulos A., Keen H., Microalbuminuria as a predictor of clinical nephropathy in insulin-dependent diabetes mellitus, Lancet, 1, pp. 1430-1432, (1982)

[6]

Mogensen C.E., Christensen C.K., Predicting diabetic nephropathy in insulin-dependent patients, N Engl J Med., 311, pp. 89-93, (1984)

[7]

Shima K., Hirota M., Fukuda M., Tanaka A., Determination of urinary lysozyme for potential detection of tubular dysfunction in diabetic nephropathy, Clin Chem., 32, 10, pp. 1818-1822, (1986)

[8]

Miltenyl M., Korner A., Tulassay T., Szabo A., Tubular dysfunction in type I diabetes mellitus, Arch Dis Child, 60, pp. 929-931, (1985)

[9]

Mutti A., Detection of renal diseases in humans: developing markers and methods, Toxicol Lett, 46, pp. 177-191, (1989)

[10]

Price R.G., Urinary enzymes, nephrotoxicity and renal disease, Toxicology, 23, pp. 99-134, (1982)

← 1 2 3 →