MITOMYCIN-C, 4-NITROQUINOLINE-1-OXIDE AND ETHYL METHANESULFONATE INDUCE LONG-LIVED LESIONS IN DNA WHICH RESULT IN SCES DURING SUCCESSIVE CELL-CYCLES IN HUMAN-LYMPHOCYTES

被引：12

作者：

DAZA, P ^{[1
]}

ESCALZA, P ^{[1
]}

MATEOS, S ^{[1
]}

CORTES, F ^{[1
]}

机构：

[1] UNIV SEVILLA,FAC BIOL,DEPT BIOL CELULAR,AVDA REINA MERCEDES S-N,E-41012 SEVILLE,SPAIN

来源：

MUTATION RESEARCH | 1992年 / 270卷 / 02期

关键词：

MITOMYCIN-C; 4-NITROQUINOLINE-1-OXIDE; ETHYL METHANESULFONATE; SISTER-CHROMATID EXCHANGE; DNA DAMAGE; 3-WAY DIFFERENTIAL STAINING; HUMAN LYMPHOCYTES;

D O I：

10.1016/0027-5107(92)90128-O

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The present study was carried out in order to analyze how persistent the lesions in DNA are which elicit sister-chromatid exchanges (SCEs), induced by three different chemical agents, mitomycin C (MMC), 4-nitroquinoline-1-oxide (4NQO) and ethyl methanesulfonate (EMS), in proliferating human lymphocytes. Cells were exposed to the mutagens for 1 h just before starting bromodeoxyuridine substitution and SCEs were examined in third-cycle metaphases showing three-way-differential staining, by means of our previously standardized method. The results show that, in spite of the fact that these three compounds have different modes of action, the lesions induced by all of them seem to be capable of persisting in DNA and eliciting SCEs for at least three successive cell cycles.

引用

页码：177 / 183

页数：7

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