RAPID QUANTITATION OF ESTROGENS BY DIAZOTIZATION AND DIRECT THIN LAYER DENSITOMETRY

被引：16

作者：

TOUCHSTONE, JC

BALIN, AK

机构：

[1] Steroid Laboratory, Department of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia

来源：

STEROIDS | 1969年 / 13卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0039-128X(69)90007-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fast Violet Salt B (6-benzoylamino-4-methoxy-5-toluidine, diazonium salt) reacts with estrogens to form stable yellow compounds. These can be separated by thin layer chromatography and quantitated directly with a double beam spectrodensitometer. The method is sensitive enough to measure 0.05 μg steroid. © 1968.

引用

页码：199 / +

页数：1

共 4 条

[1] THE INVITRO METABOLISM OF ESTROGENS BY THE LIVER STUDIED BY MEANS OF A COLORIMETRIC METHOD
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TAGNON, HJ
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[2] SCHMULOWITZ DJ, 1935, J LAB CLIN MED, V21, P210
[3] MECHANISM OF REACTION OF 4-AMINO-6-CHLORO-M-BENZENEDISULFONAMIDE WITH NITROUS ACID
URBANYI, T
PAGLIERY, M
REHM, CR
[J]. ANALYTICAL CHEMISTRY, 1967, 39 (13) : 1656 - &
[4] RAPID SENSITIVE COLORIMETRIC METHOD FOR DETERMINATION OF ESTROGENS
URBANYI, T
REHM, CR
[J]. JOURNAL OF PHARMACEUTICAL SCIENCES, 1966, 55 (05) : 501 - &

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