MECHANISM OF PHOTODYNAMIC INACTIVATION OF HUMAN-CELLS INVITRO IN THE PRESENCE OF HEMATOPORPHYRIN

被引：264

作者：

MOAN, J ^{[1
]}

PETTERSEN, EO ^{[1
]}

CHRISTENSEN, T ^{[1
]}

机构：

[1] NORWEGIAN RADIUM HOSP,NORSK HYDROS INST CANC RES,DEPT TISSUE CULTURE,OSLO,NORWAY

来源：

BRITISH JOURNAL OF CANCER | 1979年 / 39卷 / 04期

关键词：

D O I：

10.1038/bjc.1979.72

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The photosensitizing effect of haematoporphyrin (HP) on human cells of the established line NHIK 3025 has been studied. Fluorescence measurements show that HP is bound to these cells. Serum proteins also bind HP, and the presence of 10% human serum during incubation with HP (3 x 10-4M) reduces the cellular uptake of HP by 75% or more. The photosensitized inactivation is enhanced when the cells are suspended in D2O-buffer during irradiation. This indicates that singlet oxygen is involved in the inactivation. Two findings indicate that the photoinduced damage is repairable: firstly, the fraction of cells surviving a given light dose decreases with decreasing irradiation temperature, and secondly, the survival curves have a shoulder at low exposures of light. © 1979 Cancer Research Campaign.

引用

页码：398 / 407

页数：10

共 36 条

[1] RADIATION-INDUCED STRAND BREAKAGE IN DNA FROM MAMMALIAN-CELLS - STRAND SEPARATION IN ALKALINE SOLUTION [J].

AHNSTROM, G ;

ERIXON, K .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1973, 23 (03) :285-289

[2] ROLE OF LYSOSOMES AND OF CELL MEMBRANES IN PHOTOSENSITIZATION [J].

ALLISON, AC ;

MAGNUS, IA ;

YOUNG, MR .

NATURE, 1966, 209 (5026) :874-&

[3]

ALPERT B, 1976, EXCITED STATES BIOL, P425

[4]

AULER H., 1942, Z. Krebsforsck, V53, P65, DOI 10.1007/BF01792783

[5] EFFECT OF CHEMICAL-STRUCTURE ON PHOTOSENSITIZING EFFICIENCIES OF PORPHYRINS [J].

CAUZZO, G ;

GENNARI, G ;

JORI, G ;

SPIKES, JD .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1977, 25 (04) :389-395

[6] PHOTODYNAMIC EFFECT OF HEMATOPORPHYRIN THROUGHOUT THE CELL-CYCLE OF THE HUMAN CELL LINE NHIK-3025 CULTIVATED INVITRO [J].

CHRISTENSEN, T ;

MOAN, J ;

WIBE, E ;

OFTEBRO, R .

BRITISH JOURNAL OF CANCER, 1979, 39 (01) :64-68

[7] RETINAL SENSITIZED PHOTODYNAMIC DAMAGE TO LIPOSOMES [J].

DELMELLE, M .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1978, 28 (03) :357-360

[8] CELLULAR RESPONSES TO COMBINATIONS OF HYPERTHERMIA AND RADIATION [J].

DEWEY, WC ;

HOPWOOD, LE ;

SAPARETO, SA ;

GERWECK, LE .

RADIOLOGY, 1977, 123 (02) :463-474

[9]

DOUGHERTY T, 1976, RES PHOTOBIOLOGY, P435

[10]

DOUGHERTY TJ, 1978, CANCER RES, V38, P2628

← 1 2 3 4 →