RAPID-DETERMINATION OF BETA-AMINOISOBUTYRIC ACID BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：4

作者：

DEGUEVARA, OL

DENAVA, CC

PADILLA, P

ESPINOSA, J

CEBRIAN, M

GARCIA, L

机构：

[1] NATL POLYTECH INST,CTR INVEST ESTUD AVANZADOS,MEXICO CITY 14,DF,MEXICO

[2] NATL AUTONOMOUS UNIV MEXICO,INST INVEST BIOMED,MEXICO CITY 04510,DF,MEXICO

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 528卷 / 01期

关键词：

D O I：

10.1016/S0378-4347(00)82360-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

For the determination of β-aminoisobutyric acid (BAIBA) in urine samples in which the β-alanine concentrations are higher than those of BAIBA, the resolution between these two amino acids, separated by reversed-phase liquid chromatography on an octadecylsilane column, was optimized. The chromatographic analysis included precolumn derivatization of amino acids with o-phthalaldehyde, followed by a 15-min isocratic elution and detection at 340 nm. Because of its simplicity, this method should be useful for monitoring urinary excretion of BAIBA. © 1990.

引用

页码：35 / 41

页数：7

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