RESONANCE RAMAN AND ABSORPTION SPECTROSCOPIC CHARACTERIZATION OF THE CHEMICALLY ENGINEERED ENZYME THIOLSUBTILISIN - COMPARISON WITH A NATURAL THIOLENZYME

被引：6

作者：

TONGE, PJ

CAREY, PR

机构：

来源：

JOURNAL OF MOLECULAR LIQUIDS | 1989年 / 42卷

关键词：

D O I：

10.1016/0167-7322(89)80034-0

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

引用

页码：195 / 212

页数：18

共 25 条

[1] SPECTROPHOTOMETRIC INVESTIGATIONS OF MECHANISM OF ALPHA-CHYMOTRYPSIN-CATALYZED HYDROLYSES - DETECTION OF ACYL-ENZYME INTERMEDIATE [J].

BENDER, ML ;

ZERNER, B ;

SCHONBAUM, GR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1962, 84 (13) :2540-+

[2] SPECTROPHOTOMETRIC IDENTIFICATION OF ACYL ENZYME INTERMEDIATES [J].

BERNHARD, SA ;

LAU, SJ ;

NOLLER, H .

BIOCHEMISTRY, 1965, 4 (06) :1108-&

[3] EVIDENCE THAT THE LACK OF HIGH CATALYTIC ACTIVITY OF THIOLSUBTILISIN TOWARDS SPECIFIC SUBSTRATES MAY BE DUE TO AN INAPPROPRIATELY LOCATED PROTON-DISTRIBUTION SYSTEM - DEMONSTRATION OF HIGHLY NUCLEOPHILIC CHARACTER OF THE THIOL-GROUP OF THIOSUBTILISIN IN THE CATALYTICALLY RELEVANT IONIZATION STATE OF THE ACTIVE-CENTER BY USE OF A 2-PROTONIC-STATE REACTIVITY PROBECL [J].

BROCKLEHURST, K ;

MALTHOUSE, JPG .

BIOCHEMICAL JOURNAL, 1981, 193 (03) :819-823

[4] PREPARATION AND PROPERTIES OF TRANS-CINNAMOYL-PAPAIN [J].

BRUBACHER, LJ ;

BENDER, ML .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1966, 88 (24) :5871-+

[5] DIODE-ARRAY VERSUS PHOTOMULTIPLIER FOR MEASURING THE UV-EXCITED RESONANCE RAMAN-SPECTRA OF ENZYME SUBSTRATE TRANSIENTS [J].

CAREY, PR ;

CARTIER, LRS .

JOURNAL OF RAMAN SPECTROSCOPY, 1983, 14 (04) :271-275

[6]

CAREY PR, 1984, J BIOL CHEM, V259, P4357

[7]

CAREY PR, 1984, ANNU REV BIOPHYS BIO, V13, P25

[8]

CAREY PR, 1978, BIOCHEMISTRY-US, V17, P1081, DOI 10.1021/bi00599a022

[9] RESONANCE RAMAN EVIDENCE FOR SUBSTRATE REORGANIZATION IN ACTIVE-SITE OF PAPAIN [J].

CAREY, PR ;

CARRIERE, RG ;

LYNN, KR ;

SCHNEIDER, H .

BIOCHEMISTRY, 1976, 15 (11) :2387-2393

[10]

CAREY PR, 1982, BIOCH APPLICATIONS R, P169

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