IMMOBILIZATION OF FV ANTIBODY FRAGMENTS ON POROUS SILICA AND THEIR UTILITY IN AFFINITY-CHROMATOGRAPHY

被引：37

作者：

BERRY, MJ

DAVIES, J

SMITH, CG

SMITH, I

机构：

[1] Unilever Research, Sharnbrook

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 587卷 / 02期

关键词：

D O I：

10.1016/0021-9673(91)85152-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Recent advances in molecular biology have allowed antibody binding domains to be cloned and expressed in Escherichia coli. The use of Fv antibody fragments as ligands in immunoaffinity chromatography is reported. Fv fragments specific for hen-egg lysozyme were immobilized on porous silica and used to recover antigen from spiked serum in a single step. Comparison with a conventional immunoadsorbent (whole antibodies immobilized on silica) showed the Fv-silica to have a fivefold superior capacity. Analysis of sectioned Fv-silica particles by immunoelectron microscopy indicated that captured antigen was evenly distributed throughout the internal porous structure of the particle.

引用

页码：161 / 169

页数：9

共 24 条

[1] 3-DIMENSIONAL STRUCTURE OF AN ANTIGEN-ANTIBODY COMPLEX AT 2.8-A RESOLUTION
AMIT, AG
MARIUZZA, RA
PHILLIPS, SEV
POLJAK, RJ
[J]. SCIENCE, 1986, 233 (4765) : 747 - 753
[2] BERRY M J, 1991, Analytical Proceedings, V28, P141
[3] ESCHERICHIA-COLI SECRETION OF AN ACTIVE CHIMERIC ANTIBODY FRAGMENT
BETTER, M
CHANG, CP
ROBINSON, RR
HORWITZ, AH
[J]. SCIENCE, 1988, 240 (4855) : 1041 - 1043
[4] SINGLE-CHAIN ANTIGEN-BINDING PROTEINS
BIRD, RE
HARDMAN, KD
JACOBSON, JW
JOHNSON, S
KAUFMAN, BM
LEE, SM
LEE, T
POPE, SH
RIORDAN, GS
WHITLOW, M
[J]. SCIENCE, 1988, 242 (4877) : 423 - 426
[5] CLAES P, 1990, SEPARATIONS FOR BIOTECHNOLOGY 2, P611
[6] STUDIES OF POLYPEPTIDE STRUCTURE BY FLUORESCENCE TECHNIQUES .3. INTERACTION BETWEEN DYE AND MACROMOLECULE IN FLUORESCENT CONJUGATES
GILL, TJ
MCLAUGHLIN, EM
OMENN, GS
[J]. BIOPOLYMERS, 1967, 5 (03) : 297 - +
[7] HPLC ANALYSIS OF HUMAN EPIDERMAL GROWTH-FACTOR USING IMMUNOAFFINITY PRECOLUMN .1. OPTIMIZATION OF IMMUNOAFFINITY COLUMN
HAYASHI, T
SAKAMOTO, S
SHIKANABE, M
WADA, I
YOSHIDA, H
[J]. CHROMATOGRAPHIA, 1989, 27 (11-12) : 569 - 573
[8] IRWIN R, 1971, J BIOL CHEM, V246, P5329
[9] CHARACTERIZATION OF THE ERWINIA-CAROTOVORA PELB GENE AND ITS PRODUCT PECTATE LYASE
LEI, SP
LIN, HC
WANG, SS
CALLAWAY, J
WILCOX, G
[J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (09) : 4379 - 4383
[10] LIN JN, 1991, J CHROMATOGR, V542, P41

← 1 2 3 →