STUDIES ON LIMIT DEXTRINASE IN BARLEY .1. PURIFICATION OF MALT LIMIT DEXTRINASE AND PRODUCTION OF MONOSPECIFIC ANTIBODIES

Limit dextrinase from barley malt was purified to homogeneity, partially characterized and used to produce monospecific antibodies. The enzyme had a molecular weight of 104k Da with optimum activity at pH 5·5 and 55°C, and the activity was stable at 55°C for extended periods. Isoelectric focusing revealed six closely spaced bands with pl 4·2-4·6. The purified enzyme was used to immunize a rabbit and the polyclonal antiserum obtained was tested for monospecificity by immunoblotting. The antiserum contains antibodies that are monospecific to limit dextrinase. © 1992, Academic Press Limited. All rights reserved.