LACTOBACILLUS-KEFIR ALCOHOL-DEHYDROGENASE - A USEFUL CATALYST FOR SYNTHESIS

被引：197

作者：

BRADSHAW, CW

HUMMEL, W

WONG, CH

机构：

[1] Scripps Res Inst, RES INST, DEPT CHEM, 10666 N TORREY PINES RD, LA JOLLA, CA 92037 USA

[2] UNIV DUSSELDORF, INST ENZYMTECHNOL, W-4000 DUSSELDORF 1, GERMANY

[3] FORSCHUNGSZENTRUM JULICH, FORSCHUNGSZENTRUM JULICH, W-5170 JULICH 1, GERMANY

来源：

JOURNAL OF ORGANIC CHEMISTRY | 1992年 / 57卷 / 05期

关键词：

D O I：

10.1021/jo00031a037

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The alcohol dehydrogenase from Lactobacillus kefir simultaneously catalyzes carbonyl reductions and NADPH regeneration in the presence of 2-propanol. Representative synthesis of a number of chiral alcohols was accomplished in good yield and high enantiomeric excess (94-99%). This NADPH-requiring enzyme transfers the pro-R hydride from the cofactor to the si face of carbonyls to give (R) alcohols. The enzyme exhibits a very broad substrate specificity and high enantioselectivity for the synthesis of chiral aromatic, cyclic, polycyclic, and aliphatic alcohols.

引用

页码：1532 / 1536

页数：5

共 30 条

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