Fasting for less than 24 h induces cytochrome P450 2E1 and 2B1/2 activities in rats

被引：36

作者：

Brown, BL ^{[1
]}

Allis, JW ^{[1
]}

Simmons, JE ^{[1
]}

House, DE ^{[1
]}

机构：

[1] US EPA,HLTH EFFECTS RES LAB,RES TRIANGLE PK,NC 27711

来源：

TOXICOLOGY LETTERS | 1995年 / 81卷 / 01期

关键词：

CYP; 2E1; 2B1/2; F344; rats; fasting; liver microsomes; total organ activity; western blot;

D O I：

10.1016/0378-4274(95)03407-2

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

Cytochrome P450 (CYP) 2E1 activity is induced after 24 h of fasting but no information is available for shorter fasting periods. We investigated the induction of CYP 2E1, 2B1/2 and 1A1 in young adult male F344 rats after 8, 16 and 24 h of fasting compared to control. Liver microsomes were analyzed for the following enzyme activities: p-nitrophenol hydroxylase (PNP) for CYP 2E1, pentoxyresorufin-O-dealkylase (PROD) far CYP 2B1/2 and ethoxyresorufin-O-deethylase (EROD) for CYP 1A1. After each fasting interval, the activities per mg microsomal protein for PNP and PROD increased but the activity of EROD remained unchanged. Western blots for CYP 2E1 and CYP 2B1 showed increases comparable to the PNP and PROD activities, respectively. On a whole organ basis, increases were found for PNP and PROD activities, while decreases were found for EROD activity and total microsomal protein. The results are consistent with an induction of CYP 2E1 and CYP 2B1/2 activities after as little as 8 h of fasting.

引用

页码：39 / 44

页数：6

共 20 条

[1]

ALLIS JW, 1994, ANAL BIOCHEM, V218, P49

[2]

ANDERSON KE, 1991, ANNU REV NUTR, V11, P141, DOI 10.1146/annurev.nu.11.070191.001041

[3]

BURKE MD, 1974, DRUG METAB DISPOS, V2, P583

[4]

EFFECT OF CHRONIC CALORIC RESTRICTION ON PHYSIOLOGICAL VARIABLES RELATED TO ENERGY-METABOLISM IN THE MALE FISCHER-344 RAT [J].