IMMUNOLOGICALLY ACTIVE POLYSACCHARIDES .8. IMMUNOLOGICALLY ACTIVE POLYSACCHARIDES OF ARNICA-MONTANA CELL-CULTURES

被引:27
作者
PUHLMANN, J
ZENK, MH
WAGNER, H
机构
[1] UNIV MUNICH, INST PHARMACEUT BIOL, KARLSTR 29, W-8000 MUNICH 2, GERMANY
[2] COMPLEX CARBOHYDRATE RES CTR, ATHENS, GA 30602 USA
关键词
ARNICA-MONTANA; ASTERACEAE; TISSUE CULTURES; POLYSACCHARIDES; ACIDIC ARABINO-3,6-GALACTAN-PROTEIN; NEUTRAL FUCOGALACTOXYLOGLUCAN;
D O I
10.1016/S0031-9422(00)95191-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
From the nutrition medium of Arnica montana cell cultures two homogeneous polysaccharides, an acidic arabino-3,6-galactan-protein with mean M(r) of 100 000 and a neutral fucogalactoxyloglucan with mean M(r) of 22 500 have been isolated by DEAE-Sepharose CL-6B and Sephacryl S-400 column chromatography. Their structures were elucidated mainly by methylation analysis, partial acidic and enzymatic hydrolysis and C-13 NMR spectroscopy. The fucogalactoxyloglucan shows a pronounced enhancement of phagocytosis in vivo. The arabino-3,6-galactan-protein displays a strong anticomplementary effect and stimulates macrophages to excrete the tumour necrosis factor (TNF-alpha).
引用
收藏
页码:1141 / 1145
页数:5
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