INDUCTION OF GROUP-II-LIKE PHOSPHOLIPASE-A2 BY LIPOPOLYSACCHARIDE IN THE LIVER OF BCG-PRIMED RAT

被引：17

作者：

INADA, M ^{[1
]}

TOJO, H ^{[1
]}

KAWATA, S ^{[1
]}

TARUI, S ^{[1
]}

OKAMOTO, M ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT MOLEC PHYSIOL CHEM,KITA KU,OSAKA 530,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91530-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The specific activity of phospholipase A2 (PLA2) in the liver homogenate was elevated 1.7-fold in bacillus Calmette-Guerin (BCG)-treated rats, 1.6-fold in lipopolysaccharide (LPS)-treated rats, and 2.4-fold in BCG-infected rats treated with LPS, compared with that of control rats. These increased activities were almost completely inhibited by the antibody directed against rat splenic group II PLA2 (PLA2M) but not by anti-pancreatic PLA2 antibody. The results of immunoblot analysis confirmed that the PLA2 immunochemically related to the group II enzyme was induced by treatment with BCG and/or LPS. The anti-PLA2M antibody-inhibitable PLA2 activity per a single cell was elevated not only in nonparenchymal cell fraction but also in hepatocyte fraction, as in the case of whole liver. On the contrary, the PLA2 concentration and its specific activity did not change by the same treatment both in spleen homogenate and in isolated spleen cell fractions although a 3-fold increase in spleen mass occurred by BCG treatment. These results suggested that a tissue-specific mechanism of the PLA2 induction by these inflammatory mediators may operate in liver. © 1991.

引用

页码：1077 / 1083

页数：7

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