MOLECULAR-CLONING AND CHARACTERIZATION OF THE 22-KILODALTON ADULT SCHISTOSOMA-MANSONI ANTIGEN RECOGNIZED BY ANTIBODIES FROM MICE PROTECTIVELY VACCINATED WITH ISOLATED TEGUMENTAL SURFACE-MEMBRANES

被引：48

作者：

JEFFS, SA ^{[1
]}

HAGAN, P ^{[1
]}

ALLEN, R ^{[1
]}

CORREAOLIVEIRA, R ^{[1
]}

SMITHERS, SR ^{[1
]}

SIMPSON, AJG ^{[1
]}

机构：

[1] CTR PESQUISAS RENE RACHOU FIOCRUZ,BELO HORIZONTE,MG,BRAZIL

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1991年 / 46卷 / 01期

关键词：

SCHISTOSOMA; SURFACE ANTIGEN; MOLECULAR CLONING; EXPRESSION; SPECIES SPECIFICITY; PROTECTIVE IMMUNITY; VACCINE;

D O I：

10.1016/0166-6851(91)90209-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone from an adult Schistosoma mansoni lambda-gt11 expression library (A12) encoding an antigenic polypeptide of 22 kDa is described. A12 is 797 bp long and has one open reading frame encoding a protein of 190 amino acids which does not contain a signal sequence or membrane anchor motif and has no homologies with any sequences on the currently available data bases. Its product (sm22.6) is recognised by antibodies from mice protectively vaccinated with purified adult S. mansoni tegumental membranes and by serum from S. mansoni-infected Brazilians. It is present in all post-snail life cycle stages except the egg, is not sex-specific, and is found in 9 species of Schistosoma, but not in a range of other helminths. Data are presented which suggest that sm22.6 is a soluble, peripheral membrane protein.

引用

页码：159 / 168

页数：10

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