DEPENDENCE OF WHOLE-BLOOD LUMINOL CHEMILUMINESCENCE ON PMNL AND RBC COUNT

被引：11

作者：

BOCHEV, BG ^{[1
]}

MAGRISSO, MJ ^{[1
]}

BOCHEV, PG ^{[1
]}

MARKOVA, VI ^{[1
]}

ALEXANDROVA, ML ^{[1
]}

机构：

[1] MED UNIV PLEVEN,DEPT BIOPHYS,BU-5800 PLEVEN,BULGARIA

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1993年 / 27卷 / 04期

关键词：

CHEMILUMINESCENCE; WHOLE BLOOD; POLYMORPHONUCLEAR LEUKOCYTE; ERYTHROCYTE;

D O I：

10.1016/0165-022X(93)90011-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Luminol chemiluminescence (LCL) of whole blood as response to zymosan stimulation was used to assess the activity of polymorpho-nuclear leukocytes (PMNLs). Since PMNL and red blood cells (RBCs) are the primary components determining LCL response, the influence of PMNL and RBC count on the response was investigated over wide ranges of cell count and time interval. A linear relationship was found between maximum LCL intensity (I(max)) (reflecting PMNL activity) and PMNL count. This allows a proper correction of I(max) to be made as related to the sample PMNL count. This correction was valid for PMNL concentrations from 3.10(3) PMNL/ml to 6.10(5) PMNL/ml. When concentrations range from 6.10(3) PMNL/ml to 3.10(4) PMNL/ml the relationship was valid for a time interval round the peak. During the same interval the inhibitory effect of RBC count was studied. A correction of LCL was found possible for different RBC concentration ranging from 2.5.10(6) RBC/ml to 10(8) RBC/ml. These independent corrections of LCL response to PMNL and RBC counts were applied to diluted whole blood samples. The common correction of I(max) was applicable in dilutions not less than 1:50. To correct light intensity for the time interval around I(max), the dilutions used had to be not less than 1:200. The results obtained permit an objective assessment of PMNL activity to be made by testing whole blood samples from different individuals and/or samples diluted to different extents.

引用

页码：301 / 309

页数：9

共 6 条

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