BIOCHEMICAL-RESEARCH ON OOGENESIS - TRANSFER-RNA IS FULLY CHARGED IN THE 42-S STORAGE PARTICLES OF XENOPUS-LAEVIS OOCYTES

被引：17

作者：

WEGNEZ, M

DENIS, H

机构：

[1] Centre de Génétique Moléculaire, C.N.R.S, Gif-Sur-Yvette

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 98卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb13161.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transfer RNA makes up 30–40% of total RNA in previtellogenic oocytes of Xenopus laevis. The bulk of tRNA is associated with 5‐S RNA and two proteins in a high‐molecular‐weight complex sedimenting at 42 S. We show here that all kinds of tRNA are present in the 42–S particles and all of them sediment coincidently. Particle tRNA is fully charged in vivo. During purification of the 42‐S particles tRNA becomes partially uncharged. When purified particles are incubated in vitro with amino acids and ATP a charging reaction occurs without disruption of the nucleoprotein complex. Many aminoacyl‐tRNA synthetases can be shown to co‐sediment with the 42‐S particles. We conclude that complete aminoacylation of tRNA within the storage particles results from the activity of particle‐bound aminoacyl‐tRNA synthetases. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：67 / 75

页数：9

共 32 条

[1]

AGIN PA, 1977, NUCLEIC ACIDS RES, V4, P3803

[2]

AGIN PA, 1976, P NATL ACAD SCI US, V73, P3857

[3] REGULATORY SIGNIFICANCE OF TRANSFER RNA CHARGING LEVELS .I. MEASUREMENTS OF CHARGING LEVELS IN LIVERS OF CHOW-FED RATS, FASTING RATS, AND RATS FED BALANCED OR IMBALANCED MIXTURES OF AMINO ACIDS [J].