THE EFFECT OF REPLICATION ERRORS ON THE MISMATCH ANALYSIS OF PCR-AMPLIFIED DNA

被引：45

作者：

REISS, J ^{[1
]}

KRAWCZAK, M ^{[1
]}

SCHLOESSER, M ^{[1
]}

WAGNER, M ^{[1
]}

COOPER, DN ^{[1
]}

机构：

[1] UNIV LONDON KINGS COLL HOSP, SCH MED, THROMBOSIS RES UNIT, MOLEC GENET SECT, LONDON SE5 9RS, ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 04期

关键词：

D O I：

10.1093/nar/18.4.973

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mismatch analysis of PCR-ampllfied DNA has generally assumed the absence of artificially introduced base substitutions in a significant proportion of the amplification product. This technique, however, differs from the direct sequencing of amplified DNA in that non-specific substitutions will render a molecule useless in analysis. The expected signal-to-noise ratio is heavily influenced by several parameters viz, initial template copy number, number of replication cycles, eventual product yield and the type of experimental system adopted. Mathematical modelling can be used to optimize fragment length with respect to the method applied and suggests as yet undescribed improvements such as partial modification or cleavage to optimize signal detection. © 1990 Oxford University Press.

引用

页码：973 / 978

页数：6

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