MEASUREMENTS OF CYTOPLASMIC FREE CA2+ CONCENTRATION IN HUMAN PANCREATIC-ISLETS AND INSULINOMA CELLS

被引：35

作者：

KINDMARK, H

KOHLER, M

NILSSON, T

ARKHAMMAR, P

WIECHEL, KL

RORSMAN, P

EFENDIC, S

BERGGREN, PO

机构：

[1] KAROLINSKA INST, KAROLINSKA HOSP,DEPT ENDOCRINOL, ROLF LUFT CTR DIABET RES,BOX 60500, S-10401 STOCKHOLM 60, SWEDEN

[2] SODER SJUKHUSET, DEPT SURG, S-10064 STOCKHOLM, SWEDEN

[3] GOTHENBURG UNIV, DEPT MED PHYS, S-40033 GOTHENBURG, SWEDEN

来源：

FEBS LETTERS | 1991年 / 291卷 / 02期

关键词：

HUMAN PANCREATIC ISLET; HUMAN INSULINOMA; CYTOPLASMIC FREE CA2+ CONCENTRATION; FURA-2;

D O I：

10.1016/0014-5793(91)81309-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration ([Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extracellular ATP produced a transient rapid rise in [Ca2+]i. Oscillations in [Ca2+]i were observed in the presence of 10 mM glucose. Insulinoma cells responded to glucose and tolbutamide with increases in [Ca2+]i, whereas the sulphonamide diazoxide caused a decrease in [Ca2+]i. These findings confirm previous results obtained in rodent beta-cells.

引用

页码：310 / 314

页数：5

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